Immunophenotyping Kits
Primary samples used by researchers in cell and gene therapy, immunotherapy, cell line development, and immuno-oncology are unique and precious due to their biological nature. The Cellaca® PLX platform, which includes an image cytometry instrument, reagent kits, and software, is designed to increase the efficiency of sample processing, from cell staining to data readout.
The fluorescent labeled antibodies and stains in the Cellaca PLX kits are optimized for ease of use. The pre-defined assays and templates, together with the ready-to-use reagent kits enable flow-cytometry-like data analysis with no need for titrations, extra incubations, washes, or decontamination. These features permit researchers to rapidly move processed samples to downstream applications while preserving sample integrity.
Cellaca PLX Surface Marker Antibody Kits
Quickly perform cell purity checks and viability at the bench with Cellaca PLX immunophenotyping kits which combine CD3, CD4, and CD8 surface marker detection with cell viability in a single multiplex assay.
Data from Cellaca PLX System was exported to FCS Express™ Flow Cytometry software for population analysis. Human PBMCs were labeled with anti-CD3 KIRAVIA Blue 520™ and Hoechst. A gate was set on cells that were CD3 and Hoechst positive (shown in A). Further population analysis showed CD3 positive cells that were also CD4-PE and CD8-APC positive (shown in B).
Single Color Antibodies
Single color antibodies and isotype controls have been optimized for labeling PBMCs, PBMCs with red blood-cell contamination, and cultured cell lines.
| Description | Catalog Number | Unit Size | Manual |
|---|---|---|---|
| KIRAVIA Blue 520™ anti-human CD3 Antibody* | CS1-A0001-1 | 25 tests | |
| CS1-A0001-2 | 100 tests | ||
| Cellaca® PLX, anti-human CD3 PE Antibody | CS1-A0002-1 | 25 tests | |
| CS1-A0002-2 | 100 tests | ||
| Cellaca® PLX, anti-human CD3 APC Antibody | CS1-A0003-1 | 25 tests | |
| CS1-A0003-2 | 100 tests | ||
| Cellaca® PLX, anti-human CD4 PE Antibody | CS1-A0008-1 | 25 tests | |
| CS1-A0008-2 | 100 tests | ||
| Cellaca® PLX, anti-human CD4 APC Antibody | CS1-A0009-2 | 100 tests | |
| Cellaca® PLX, anti-human CD8 PE Antibody | CS1-A0012-1 | 25 tests | |
| CS1-A0012-2 | 100 tests | ||
| Cellaca® PLX, anti-human CD8 APC Antibody | CS1-A0014-1 | 25 tests | |
| CS1-A0014-2 | 100 tests |
Isotype Controls
| Description | Catalog Number | Unit Size | Manual |
|---|---|---|---|
| KIRAVIA Blue 520™ Mouse IgG1, κ Isotype Ctrl Antibody* | CS1-A0004-1 | 25 tests | |
| CS1-A0004-2 | 100 tests | ||
| Cellaca® PLX, PE Mouse IgG2a, κ Isotype Ctrl Antibody | CS1-A0005-1 | 100 tests | |
| Cellaca® PLX, APC Mouse IgG2a, κ Isotype Ctrl Antibody | CS1-A0006-1 | 100 tests | |
| Cellaca® PLX, PE Mouse IgG1, κ Isotype Ctrl (FC) Antibody | CS1-A0013-1 | 25 tests | |
| CS1-A0013-2 | 100 tests | ||
| Cellaca® PLX, APC Mouse IgG1, κ Isotype Ctrl Antibody | CS1-A0011-1 | 100 tests |
*KIRAVIA Blue 520™ is a trademark of Sony. This product is subject to proprietary rights of Sony and is made and sold under license from Sony Corporation.
Single Surface Marker Viability Kits
Single surface marker kits contain a conjugated t-cell receptor antibody, a corresponding isotype control, a dead cell identification dye, and a total cell stain.
| Description | Catalog Number | Unit Size | Manual |
|---|---|---|---|
| Cellaca® PLX, anti-human CD3 KB520 Viability Kit | CSK-A0001-1 | 25 tests | |
| CSK-A0001-2 | 100 tests | ||
| Cellaca® PLX, anti-human CD3 PE Viability Kit | CSK-A0002-1 | 25 tests | |
| CSK-A0002-2 | 100 tests | ||
| Cellaca® PLX, anti-human CD3 APC Viability Kit | CSK-A0003-1 | 25 tests | |
| CSK-A0003-2 | 100 tests | ||
| Cellaca® PLX, anti-human CD4 PE Viability Kit | CSK-A0004-1 | 25 tests | |
| CSK-A0004-2 | 100 tests | ||
| Cellaca® PLX, anti-human CD4 APC Viability Kit | CSK-A0005-2 | 100 tests | |
| Cellaca® PLX, anti-human CD8 PE Viability Kit | CSK-A0006-1 | 25 tests | |
| CSK-A0006-2 | 100 tests | ||
| Cellaca® PLX, anti-human CD8 APC Viability Kit | CSK-A0007-1 | 25 tests | |
| CSK-A0007-2 | 100 tests |
Two Surface Marker Total Cell Kits
These kits contain a combination of two conjugated t-cell antibodies, associated isotype controls, and total cell dye for identification of nucleated cells. The kits have been designed to work with samples that may contain RBC contamination with the PBMC sample.
| Description | Catalog Number | Unit Size | Manual |
|---|---|---|---|
| Cellaca® PLX, anti-human CD3 KB520/CD4 APC Total Cell Kit | CSK-A0008-2 | 100 tests | |
| Cellaca® PLX, anti-human CD3 KB520/CD8 APC Total Cell Kit | CSK-A0009-1 | 25 tests | |
| CSK-A0009-2 | 100 tests | ||
| Cellaca® PLX, anti-human CD3 APC/CD4 PE Total Cell Kit | CSK-A0012-1 | 25 tests | |
| CSK-A0012-2 | 100 tests | ||
| Cellaca® PLX, anti-human CD3 APC/CD8 PE Total Cell Kit | CSK-A0013-1 | 25 tests | |
| CSK-A0013-2 | 100 tests | ||
| Cellaca® PLX, anti-human CD4 PE/CD8 APC Total Cell Kit | CSK-A0014-1 | 25 tests | |
| CSK-A0014-2 | 100 tests |
Two Surface Marker Dead Cell Kits
These kits contain a combination of two conjugated t-cell antibodies, associated isotype controls, and a dead cell identification dye. The kits have been designed to work with cell line samples and PBMC samples that have undergone a RBC lysis step and therefore do not have red blood cells in the samples.
| Description | Catalog Number | Unit Size | Manual |
|---|---|---|---|
| Cellaca® PLX, anti-human CD3 KB520/CD4 APC Dead Cell Kit | CSK-A0016-2 | 100 tests | |
| Cellaca® PLX, anti-human CD3 KB520/CD8 APC Dead Cell Kit | CSK-A0017-1 | 25 tests | |
| CSK-A0017-2 | 100 tests | ||
| Cellaca® PLX, anti-human CD3 APC/CD4 PE Dead Cell Kit | CSK-A0020-1 | 25 tests | |
| CSK-A0020-2 | 100 tests | ||
| Cellaca® PLX, anti-human CD3 APC/CD8 PE Dead Cell Kit | CSK-A0021-1 | 25 tests | |
| CSK-A0021-2 | 100 tests | ||
| Cellaca® PLX, anti-human CD4 PE/CD8 APC Dead Cell Kit | CSK-A0022-1 | 25 tests | |
| CSK-A0022-2 | 100 tests |
Two Surface Marker Viability Kits
These kits contain a combination of two conjugated t-cell antibodies, associated isotype controls, and a dead cell identification dye, and a total cell dye for identification of nucleated cells. The kits can be used with cultured cell lines, PBMCs, and PBMCs with red blood-cell contamination.
Three Surface Marker Total Cell Kits
These kits contain a combination of three conjugated t-cell antibodies, associated isotype controls, and total cell dye for identification of nucleated cells. The kits have been designed to work with samples that may contain RBC contamination with the PBMC sample.
Three Surface Marker Dead Cell Kits
These kits contain a combination of three conjugated t-cell antibodies, associated isotype controls, and a dead cell identification dye. The kits have been designed to work with cell line samples and PBMC samples that have undergone a RBC lysis step and therefore do not have red blood cells in the samples.